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To confirm that the MONTS 16S rRNA sequences obtained in our libraries originated from Efs and Eds, we applied fluorescence in situ hybridization (FISH) probes specifically targeting those sequences (probe Efs1057 and Eds214, respectively) to whole mount Eubostrichus nematodes. All bacteria attached to the host surface were triple stained by the bacterial probe EUB338, by the Gammaproteobacteria specific probe GAM42a and by the respective MONTS specific probes (Fig. 3 and Supplementary Fig.

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